Review



moclo tool kit  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    Addgene inc moclo tool kit
    Moclo Tool Kit, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 84 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tool-kit/MoClo+Toolkit+(Kit+%231000000044)/pmc12868398-157-10-15
    Average 95 stars, based on 84 article reviews
    moclo tool kit - by Bioz Stars, 2026-11
    95/100 stars

    Images

    Related Articles

    Cloning:

    Article Title: In planta complementation of the betalain biosynthetic pathway with a bacterial dioxygenase.
    Article Snippet: .. Cloning components were acquired from the MoClo Plant Tool Kit and the MoClo Plant Parts Kit (Addgene, Cambridge, MA, USA) and coding sequences of genes (S1 Table) involved in the betalain pathway were codon optimised for their expression in N. benthamiana. ..

    Article Title: In planta complementation of the betalain biosynthetic pathway with a bacterial dioxygenase
    Article Snippet: .. Cloning components were acquired from the MoClo Plant Tool Kit and the MoClo Plant Parts Kit (Addgene, Cambridge, MA, USA) and coding sequences of genes ( ) involved in the betalain pathway were codon optimised for their expression in N. benthamiana . ..

    Article Title: Quantitative profiling of millions of nucleotides reveals sequence-encoded interactions that govern plasmid propagation
    Article Snippet: .. Single-part plasmid libraries were assembled using the Mammalian Tool Kit (MTK) modular cloning framework, sourced from Addgene. ( ) Individual regulatory elements (promoters, UTRs, coding sequences, localization tags, and polyadenylation sequences) were cloned into a common bacterial backbone containing a chloramphenicol resistance marker and a pMB1-derived origin of replication. .. Multi-part expression cassettes were assembled by one-pot Golden Gate Type IIS assembly using BsaI-HFv2 (NEB) and T4 DNA Ligase (NEB) according to the MTK assembly format, with reaction cleanup performed by SPRI bead purification.

    Article Title: Arogenate dehydrogenase polynucleotides, polypeptides and methods of using the same
    Article Snippet: Transcripts were amplified by PCR using gene specific primers (Table S1) and Phusion High-Fidelity DNA polymerase (Thermo Fisher Scientific, Waltham, MA, USA). .. Vectors for transient transformation were constructed with Golden Gate cloning using the MoClo Tool Kit (Weber et al., 2011; Addgene, Cambridge, MA, USA), with the BpiI and BsaI restriction sites eliminated after cloning. .. The turboGFP sequence used in this assay was a variant codon-optimized for plants contained in the MoClo Plant Parts Kit (Engler et al., 2014; Addgene, Cambridge, MA, USA).

    Expressing:

    Article Title: In planta complementation of the betalain biosynthetic pathway with a bacterial dioxygenase.
    Article Snippet: .. Cloning components were acquired from the MoClo Plant Tool Kit and the MoClo Plant Parts Kit (Addgene, Cambridge, MA, USA) and coding sequences of genes (S1 Table) involved in the betalain pathway were codon optimised for their expression in N. benthamiana. ..

    Article Title: In planta complementation of the betalain biosynthetic pathway with a bacterial dioxygenase
    Article Snippet: .. Cloning components were acquired from the MoClo Plant Tool Kit and the MoClo Plant Parts Kit (Addgene, Cambridge, MA, USA) and coding sequences of genes ( ) involved in the betalain pathway were codon optimised for their expression in N. benthamiana . ..

    Amplification:

    Article Title: Model-Driven Engineering of Yarrowia lipolytica for Improved Microbial Oil Production.
    Article Snippet: Amplified parts were cloned into the pCR- Blunt vector using Zero Blunt PCR Cloning Kit by following the instructions from the supplier (Invitrogen, Waltham, MA). .. These amplified parts were assembled with the parts from the Yarrowia lipolytica Golden Gate tool kit (Addgene kit #1000000167) by facilitating BsaI restriction sites; promoter (pCR4Blunt- TOPO- P1 TEF- 8UAS), terminator (pCR4Blunt- TOPO- TLip2 (E- L)), two markers for antibiotic resistance (hygromycin, pCR4Blunt- TOPO- M- hph, and nourseothricin, pCR4Blunt- TOPO- M- Nat), MFE homologous sites with NotI restriction sites (pCR4Blunt- TOPO- MFE- NotI_Up, pCR4Blunt- TOPO- MFE- NotI_Down) and backbone plasmid (pSB1A3) (Larroude et al. 2019). .. Assembled plasmids were transformed into E. coli Zymo 10B cells (Cat #T3020; Zymo Research, Irvine, CA, The US) by following the supplier's instructions, and the transformed strains were stored at −80°C.

    Article Title: Model‐Driven Engineering of Yarrowia lipolytica for Improved Microbial Oil Production
    Article Snippet: Amplified parts were cloned into the pCR‐Blunt vector using Zero Blunt PCR Cloning Kit by following the instructions from the supplier (Invitrogen, Waltham, MA). .. These amplified parts were assembled with the parts from the Yarrowia lipolytica Golden Gate tool kit (Addgene kit #1000000167) by facilitating BsaI restriction sites; promoter (pCR4Blunt‐TOPO‐ P1 TEF‐8UAS), terminator (pCR4Blunt‐TOPO‐TLip2 (E‐L)), two markers for antibiotic resistance (hygromycin, pCR4Blunt‐TOPO‐M‐hph, and nourseothricin, pCR4Blunt‐TOPO‐M‐Nat), MFE homologous sites with NotI restriction sites (pCR4Blunt‐TOPO‐MFE‐NotI_Up, pCR4Blunt‐TOPO‐MFE‐NotI_Down) and backbone plasmid (pSB1A3) (Larroude et al. ). .. Assembled plasmids were transformed into E. coli Zymo 10B cells (Cat #T3020; Zymo Research, Irvine, CA, The US) by following the supplier's instructions, and the transformed strains were stored at −80°C.

    Plasmid Preparation:

    Article Title: Model-Driven Engineering of Yarrowia lipolytica for Improved Microbial Oil Production.
    Article Snippet: Amplified parts were cloned into the pCR- Blunt vector using Zero Blunt PCR Cloning Kit by following the instructions from the supplier (Invitrogen, Waltham, MA). .. These amplified parts were assembled with the parts from the Yarrowia lipolytica Golden Gate tool kit (Addgene kit #1000000167) by facilitating BsaI restriction sites; promoter (pCR4Blunt- TOPO- P1 TEF- 8UAS), terminator (pCR4Blunt- TOPO- TLip2 (E- L)), two markers for antibiotic resistance (hygromycin, pCR4Blunt- TOPO- M- hph, and nourseothricin, pCR4Blunt- TOPO- M- Nat), MFE homologous sites with NotI restriction sites (pCR4Blunt- TOPO- MFE- NotI_Up, pCR4Blunt- TOPO- MFE- NotI_Down) and backbone plasmid (pSB1A3) (Larroude et al. 2019). .. Assembled plasmids were transformed into E. coli Zymo 10B cells (Cat #T3020; Zymo Research, Irvine, CA, The US) by following the supplier's instructions, and the transformed strains were stored at −80°C.

    Article Title: Quantitative profiling of millions of nucleotides reveals sequence-encoded interactions that govern plasmid propagation
    Article Snippet: .. Single-part plasmid libraries were assembled using the Mammalian Tool Kit (MTK) modular cloning framework, sourced from Addgene. ( ) Individual regulatory elements (promoters, UTRs, coding sequences, localization tags, and polyadenylation sequences) were cloned into a common bacterial backbone containing a chloramphenicol resistance marker and a pMB1-derived origin of replication. .. Multi-part expression cassettes were assembled by one-pot Golden Gate Type IIS assembly using BsaI-HFv2 (NEB) and T4 DNA Ligase (NEB) according to the MTK assembly format, with reaction cleanup performed by SPRI bead purification.

    Article Title: Model‐Driven Engineering of Yarrowia lipolytica for Improved Microbial Oil Production
    Article Snippet: Amplified parts were cloned into the pCR‐Blunt vector using Zero Blunt PCR Cloning Kit by following the instructions from the supplier (Invitrogen, Waltham, MA). .. These amplified parts were assembled with the parts from the Yarrowia lipolytica Golden Gate tool kit (Addgene kit #1000000167) by facilitating BsaI restriction sites; promoter (pCR4Blunt‐TOPO‐ P1 TEF‐8UAS), terminator (pCR4Blunt‐TOPO‐TLip2 (E‐L)), two markers for antibiotic resistance (hygromycin, pCR4Blunt‐TOPO‐M‐hph, and nourseothricin, pCR4Blunt‐TOPO‐M‐Nat), MFE homologous sites with NotI restriction sites (pCR4Blunt‐TOPO‐MFE‐NotI_Up, pCR4Blunt‐TOPO‐MFE‐NotI_Down) and backbone plasmid (pSB1A3) (Larroude et al. ). .. Assembled plasmids were transformed into E. coli Zymo 10B cells (Cat #T3020; Zymo Research, Irvine, CA, The US) by following the supplier's instructions, and the transformed strains were stored at −80°C.

    Clone Assay:

    Article Title: A modular synthetic biology toolkit unlocks metabolic engineering of the industrially relevant alga Nannochloropsis
    Article Snippet: For plasmid construction and assembly, a molar ratio of 1:3 (insert-to-vector) was routinely applied. .. The parts were cloned into the level 0 vectors of MoClo Tool Kit (Kit #1000000044; Addgene). ..

    Article Title: Quantitative profiling of millions of nucleotides reveals sequence-encoded interactions that govern plasmid propagation
    Article Snippet: .. Single-part plasmid libraries were assembled using the Mammalian Tool Kit (MTK) modular cloning framework, sourced from Addgene. ( ) Individual regulatory elements (promoters, UTRs, coding sequences, localization tags, and polyadenylation sequences) were cloned into a common bacterial backbone containing a chloramphenicol resistance marker and a pMB1-derived origin of replication. .. Multi-part expression cassettes were assembled by one-pot Golden Gate Type IIS assembly using BsaI-HFv2 (NEB) and T4 DNA Ligase (NEB) according to the MTK assembly format, with reaction cleanup performed by SPRI bead purification.

    Article Title: A modular synthetic biology toolkit unlocks metabolic engineering of the industrially relevant alga Nannochloropsis.
    Article Snippet: For plasmid construction and assembly, a molar ratio of 1:3 (insert-to-vector) was routinely applied. .. The parts were cloned into the level 0 vectors of MoClo Tool Kit (Kit #1000000044; Addgene). ..

    Marker:

    Article Title: Quantitative profiling of millions of nucleotides reveals sequence-encoded interactions that govern plasmid propagation
    Article Snippet: .. Single-part plasmid libraries were assembled using the Mammalian Tool Kit (MTK) modular cloning framework, sourced from Addgene. ( ) Individual regulatory elements (promoters, UTRs, coding sequences, localization tags, and polyadenylation sequences) were cloned into a common bacterial backbone containing a chloramphenicol resistance marker and a pMB1-derived origin of replication. .. Multi-part expression cassettes were assembled by one-pot Golden Gate Type IIS assembly using BsaI-HFv2 (NEB) and T4 DNA Ligase (NEB) according to the MTK assembly format, with reaction cleanup performed by SPRI bead purification.

    Transformation Assay:

    Article Title: Arogenate dehydrogenase polynucleotides, polypeptides and methods of using the same
    Article Snippet: Transcripts were amplified by PCR using gene specific primers (Table S1) and Phusion High-Fidelity DNA polymerase (Thermo Fisher Scientific, Waltham, MA, USA). .. Vectors for transient transformation were constructed with Golden Gate cloning using the MoClo Tool Kit (Weber et al., 2011; Addgene, Cambridge, MA, USA), with the BpiI and BsaI restriction sites eliminated after cloning. .. The turboGFP sequence used in this assay was a variant codon-optimized for plants contained in the MoClo Plant Parts Kit (Engler et al., 2014; Addgene, Cambridge, MA, USA).

    Construct:

    Article Title: Arogenate dehydrogenase polynucleotides, polypeptides and methods of using the same
    Article Snippet: Transcripts were amplified by PCR using gene specific primers (Table S1) and Phusion High-Fidelity DNA polymerase (Thermo Fisher Scientific, Waltham, MA, USA). .. Vectors for transient transformation were constructed with Golden Gate cloning using the MoClo Tool Kit (Weber et al., 2011; Addgene, Cambridge, MA, USA), with the BpiI and BsaI restriction sites eliminated after cloning. .. The turboGFP sequence used in this assay was a variant codon-optimized for plants contained in the MoClo Plant Parts Kit (Engler et al., 2014; Addgene, Cambridge, MA, USA).



    Similar Products

    94
    Cytiva Europe open source reconstruction toolkit
    Open Source Reconstruction Toolkit, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tool-kit/Tool+Kit/pmc13595413-67-10-20
    Average 94 stars, based on 1 article reviews
    open source reconstruction toolkit - by Bioz Stars, 2026-11
    94/100 stars
      Buy from Supplier

    99
    ATCC adipocyte differentiation tool kit
    Adipocyte Differentiation Tool Kit, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tool-kit/Adipocyte+Differentiation+Toolkit+for+Adipose-Derived+MSCs+and+Preadipocytes/pm41764532-52-15-19
    Average 99 stars, based on 1 article reviews
    adipocyte differentiation tool kit - by Bioz Stars, 2026-11
    99/100 stars
      Buy from Supplier

    94
    Cytiva Europe dosimetry toolkit
    Dosimetry Toolkit, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tool-kit/Tool+Kit/pmc13246971-318-9-11
    Average 94 stars, based on 1 article reviews
    dosimetry toolkit - by Bioz Stars, 2026-11
    94/100 stars
      Buy from Supplier

    99
    Qiagen dneasy powersoil tool kit
    Dneasy Powersoil Tool Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tool-kit/DNeasy+PowerSoil+Pro+Kit/pm41775257-514-11-15
    Average 99 stars, based on 1 article reviews
    dneasy powersoil tool kit - by Bioz Stars, 2026-11
    99/100 stars
      Buy from Supplier

    99
    New England Biolabs nebuilder assembly tool
    Nebuilder Assembly Tool, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tool-kit/NEBuilder+HiFi+DNA+Assembly+Cloning+Kit/pmc12886786-362-11-11
    Average 99 stars, based on 1 article reviews
    nebuilder assembly tool - by Bioz Stars, 2026-11
    99/100 stars
      Buy from Supplier

    86
    Ecogenomics Inc genome taxonomy database tool kit gtdb tk v2 4 1
    Genome Taxonomy Database Tool Kit Gtdb Tk V2 4 1, supplied by Ecogenomics Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tool-kit/database+genome+taxonomy/pm41634308-232-18-25
    Average 86 stars, based on 1 article reviews
    genome taxonomy database tool kit gtdb tk v2 4 1 - by Bioz Stars, 2026-11
    86/100 stars
      Buy from Supplier

    95
    Addgene inc moclo tool kit
    Moclo Tool Kit, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tool-kit/MoClo+Toolkit+(Kit+%231000000044)/pmc12868398-157-10-15
    Average 95 stars, based on 1 article reviews
    moclo tool kit - by Bioz Stars, 2026-11
    95/100 stars
      Buy from Supplier

    Image Search Results